Fish
FISH (Fluorescent In Situ Hybridization) is a molecular biology technique used to identify and locate specific DNA sequences within chromosomes, and in the context of semen, it is employed to assess the presence of chromosomal abnormalities in sperm cells.
The results of the test therefore provide information on the percentage of sperm cells with chromosomal abnormalities and are useful for determining the likelihood of success of assisted reproduction techniques and for identifying any risks of transmitting genetic abnormalities to offspring.
The FISH and TUNEL tests on semen provide crucial information about sperm quality and male reproductive health.
These tests are particularly useful for couples facing infertility issues and can guide clinical decisions regarding assisted reproduction treatments.
FISH
Available panels
FISH on Semen
FISH
Who it's recommended for
The FISH test is indicated in the following cases:
Unexplained infertility
History of recurrent miscarriages
Failure of in vitro fertilization (IVF) cycles
Diagnosis of hereditary genetic conditions
FISH
Analysis method
The patient provides a semen sample through masturbation, collected in a sterile container.
Sperm cells are isolated from the semen and prepared for analysis.
Fluorescent probes specific to the chromosomes of interest (e.g., X, Y, 13, 15, 16, 18, 21, 22) are used. These probes bind to the corresponding DNA sequences in the chromosomes.
The sample is examined under a fluorescence microscope to identify and count chromosomal abnormalities.